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Institute of Neuroscience, NYMU
Tsai-Wen Chen Assistant Professor

 

Tsai-Wen Chen Assistant Professor

chentw@ym.edu.tw

Tel : +886-2-2826-7102

PhD Neuroscience,
University of Göttingen, Germany
Laboratory of Brain Connectivity
 
B.S., Electrical Engineering, National Taiwan University

 

Education/Experience

2010-2015 Postdoc, Janelia Research Campus, USA

2008-2010 Postdoc, University of Göttingen, Germany

2003-2008 PhD Neuroscience, University of Göttingen, Germany

1997-2001 B.S., Electrical Engineering, National Taiwan University

 

相關報導

神經科學新技術-如何解開大腦的神祕訊息?科學月刊2016年7月號

Tsai-Wen Chen CV

Google Scholar

 

Research Interest

I am interested in how the cerebral cortex mediates our mental functions. We use two-photon calcium imaging to probe cortical circuits while animals perform cognitive tasks. We also engineer microscopes and protein sensors to improve visualization of cortical function.

本實驗室運用先進的光學顯微技術與螢光分子探針即時地觀測腦中神經網路的訊息處理(Li, Chen et. al. 2015 Nature)。同時,我們也致力發展探索大腦的新科技:包括提升螢光分子探針敏感度 (Chen el. al. 2013 Nature),研發處理神經影像的演算法(Chen et. al. 2006 Biophys. J. ),以及發展超高速的光學顯微技術(Junek and Chen et. al. 2008) 等 。

目前實驗室研究的方向包括:

  1.運用雙光子鈣離子影像捕捉小鼠腦中記憶形成的過程(與神研所林貝容老師合作)。

 海馬迴與大腦皮質是記憶形成與長期儲存的關鍵腦區。運用雙光子鈣離子影像,我們在活體小鼠的腦中

 同時紀錄數百顆神經元的活性,希望藉此解讀記憶形成時的神經密碼。

  2.發展活體神經電壓影像的關鍵技術。

 神經細胞透過細胞膜電位進行運算並傳遞訊息,可是目前仍沒有任何技術能在活體動物的腦中高速偵

 測大量神經元的細胞內膜電位訊號。我們正致力研發活體神經電壓影像的關鍵技術。包括電壓敏感蛋白

 的篩選測試、高速影像系統的開發、以及巨量資料分析的平台等。

  3.發展大規模追蹤神經連結強度的新技術。

 神經網路連結強度的變化與人類學習記憶的能力息息相關。同時,許多的精神疾病,包括知覺失調症

 等,也與大腦細胞間的不正常連結有關。我們計畫結合光遺傳學刺激與神經影像記錄,發展在活體腦中

 大規模追蹤神經網路連結強度的嶄新技術。

 

Invited Lectures/Publications

Chen T-W, Li N, Daie K, Svoboda K (2017) A map of anticipatory activity in mouse motor cortex. Neuron, 94, 866-879

Li N, Chen T-W, Guo Z, Gerfen C, Svoboda K (2015) A motor cortex circuit for motor planning and movement. Nature, 519 (7541) 51-56.

Dana H, Chen T-W, Hu A, Shields B, Guo C, Looger L, Kim D, Svoboda K (2014) Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo PloS One, 9 (9)

Chen T-W, Wardill T, Sun Y, Pulver S, Renninger S, Baohan A, Schreiter E, Kerr R, Orger M, Jayaraman V, Looger LL, Svoboda K, Kim D (2013) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature, 499 (7458) 295-300

Akerboom J*, Chen T-W*, Wardill T, Tian L, Marvin J, Mutlu S, Calderón N, Esposti F, Borghuis B, Sun X, Gordus A, Orger M, Portugues R, Engert F, Macklin J, Filosa A, Aggarwal A, Kerr R, Takagi R, Kracun S, Shigetomi E, Khakh B, Baier H, Lagnado L, Wang S, Bargmann C, Kimmel B, Jayaraman V, Svoboda K, Kim D, Schreiter E, and Looger L (2012) Optimization of a GCaMP calcium indicator for neural activity imaging. J. Neurosci. 32(40) 13819-40 (*equal contribution)

Zariwala H, Borghuis B, Hoogland T, Madisen L, Tian L, DeZeeuw C, Zeng H, Looger L, Svoboda K, and Chen T-W* (2012) A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo. J. Neurosci. 32(9):3131-41 (*corresponding author)

Junek S*, Chen T-W*, Alevra M, and Schild D (2009) Activity correlation imaging: visualizing function and structure of neuronal populations. Biophys. J. 96, 3801-3809 (*equal contribution)

Chen T-W*, Lin B-J*, and Schild D (2009) Odor coding by modules of coherent mitral/tufted cells in the vertebrate olfactory bulb. Proc. Natl. Acad. Sci. U S A. 106, 2401-2406 (*equal contribution)

Chen, T-W, Lin B-J, Brunner E, and Schild D (2006). In situ background estimation in quantitative fluorescence imaging. Biophys. J. 90, 2534-2547

 

 

 

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